Coding

Part:BBa_K3192019

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-12)


styE

This part contains the styE gene taken from Pseudomonas putida S12 . This encodes the putative styrene transporter protein. This protein works to help shuttle the styrene molecules across the cell membrane into the cytoplasm to begin styrene degradation. It contains a 24 residue peptide for signaling to transport styrene. This is vital in the gene cluster styABCDE to allow the cell to metabolize styrene.1

References:

1) Mooney, A., Oleary, N. D. & Dobson, A. D. W. Cloning and Functional Characterization of the styE Gene, Involved in Styrene Transport in Pseudomonas putida CA-3. Applied and Environmental Microbiology 72,1302–1309 (2006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1040
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 125


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Parameters
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