Part:BBa_K3170999

FRET system for detecting TEVp activity

We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp.

Experimental Results

• Imaging begins approximately 2 hours after the media is replaced with a fluorescent imaging medium. Get images every 1 hour. SW1990 cells were observed using 440 nm excitation light, and the cells turned from cyan to yellow.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 694
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]