Composite
Part:BBa_K3163002
Designed by: Burhanuddin Sabuwala, Neha Swaminathan, Shashank H S Group: iGEM19_IIT-Madras (2019-10-16)
CHARACTERISATION
We created a part ( BBa_K3163002) such that eGFP is driven by a CaMV 35s Promoter and flanked by NOS terminator which is already present in pCAMBIA 1301. We cloned this part into pCAMBIA which replicates in Fusarium solani and E. coli. Here, we have transformed the plasmid in to E. coli TOP 10 and performed fluorometry studies to confirm the presence of GFP. analysis. Higher fluorescence in the culture containing our part confirmed the presence of GFP and this was found to be statistically significant by ANOVA analysis. Characterisation of BBa_K3163002 in E. coli is reported in the figure given below.
Figure : Normalized fluorescence with respect to Empty Vector (pCAMBIA 1301) in E. coli.
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