LytC cell wall anchoring protein for B. subtilis
The cell wall binding domain of lytC, a N-acetylmuramoyl-L-alanine amidase of B. subtilis, was isolated to be used as a cell wall anchor. As demonstrated by Kobayashi et al. 2000 this domain can be used to target catalytic domians of other proteins as well as peptides to the cell wall of B. subtilis whilst leaving their functionality intact.
This biobrick contains 2-953 of 1491bp of the complete genomic LytC. Full sequence can be found on EMBL (Acession number AL009126.3)
Figure I. Graphical representation of cell wall binding protein.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
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