Reporter

Part:BBa_K315003

Designed by: Tom Shuman   Group: iGEM10_Davidson-MissouriW   (2010-06-16)


pLac+RBS+TetA+RBS+RFP

This construct was built to determine if there is a transcription terminator in the 3' end of the TetA gene. An absence of RFP expression despite this strong promoter would point to the presence of such a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 370
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 516
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 542
    Illegal NgoMIV site found at 910
    Illegal NgoMIV site found at 1070
    Illegal AgeI site found at 1998
    Illegal AgeI site found at 2110
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLac+RBS+TetA+RBS+RFP