RBS

Part:BBa_K3136012:Design

Designed by: Shiyuan Li   Group: iGEM19_Shanghai_HS_United   (2019-09-18)


B0034-RBS-C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a mutation sequence from B0034 RBS, and it is constructed in a typeIIS plasmid. We know that the scar between RBS and coding sequence would effect the protein expression, so we construct several different RBSs to test the expression strength.


Source

It is a synthetic sequence.

References