RBS

Part:BBa_K3105111:Design

Designed by: Katherin Vallejos Torres   Group: iGEM19_Uppsala_Universitet   (2019-08-26)


RBS with ribosome standby site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

8 CA repeats were added to flanken the wing region of the RBS.


Source

Ribosome standby site sequence was retrieved from Sterk et al. 2017 "Unstructured 5′-tails act through ribosome standby to override inhibitory structure at ribosome binding sites". This sequence was added to the original part B0034.

References