Composite
Part:BBa_K3042006
Designed by: Rehmat Babar Group: iGEM19_Georgia_State (2019-10-16)
Dino III RFP Plasmid
DinoIII is a dinoflagellate expression system that was constructed on the basis of genomic and transcriptic data from many dinoflagellates and created by the University of Connecticut. DinoIII- RFP is a dinoflagellate optimized reporter plasmid that contains a red fluorescence protein. Designed by GSU’s iGEM team, it will be used to transform into the dinoflagellate, O.Marina. The original Dino III vector included a green fluorescence protein which for O.marina, a heterotrophic dinoflagellate, is not an issue. However, because we plan to transform Symbiodinium with this composite part, RFP was designated to replace GFP to prevent inhibition of the Symbiodinium’s photosynthetic abilities.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 693
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1764
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1376
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 693
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 693
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1746
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Categories
Parameters
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