Coding

Part:BBa_K3042004

Designed by: Rehmat Babar   Group: iGEM19_Georgia_State   (2019-10-11)


Dinoflagellate Codon Optimized GFP

GFP (green fluorescence protein) comes from crystal jelly, Aequorea Victoria, a bioluminescent jellyfish. Aequorea victoria gfp was amplified from a pGloTM Plasmid (Bio-Rad, Hercules, CA, USA). The pGLO plasmid contains gfp and other DNA sequences for gene expression and bacterial transformations.The gfp part contains a SpeI site at 5’-end and a BclI site at 3’-end. This sequence was derived from the University of Connecticut.

Primers needed:
gfpNF2- AACTAGTATGGCTAGCAAAGGAGAAGAACTTTTC
gfpNR- TATGATCATCATTTGTAGAGCTCATCCATGC


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 523
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None