Translational_Unit

Part:BBa_K302034

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)

mazEF toxin-antitoxin cluster

Encodes a stable non-specific ribonuclease toxin (mazF) and its inhibitory antitoxin (mazE) in Bacillus Subtilis. These genes are used in Bacillus Subtilis to provide a toxin-antitoxin kill switch in various stressful conditions. When expression of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.

Other Uses

This toxin-antitoxin system has been improved and adapted for different uses twice so far. The most effective use for a long term bioengineering purpose can be seen in part:BBa_K3588014 or at https://2020.igem.org/Team:CLS_CLSG_UK/Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None