Cell

Part:BBa_K300984:Design

Designed by: Manuel Lupotto, Lorenzo Pasotti, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)

E. coli strain BW25141 (pir+)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is not the only strain able to propagate R6K at medium copy, but it has been mentioned in several papers and it is easily retrievable from the CGSC.


Source

Coli Genetic Stock Center: strain number CGSC#7635.

References

[1] Haldimann A, Wanner BL (2001), Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. Journal of Bacteriology, 183(21), p.6386-6393.

[2] Coli Genetic Stock Center (Yale University), strain code CGSC#7635.

[3] Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, Keasling JD (2010), BglBricks: A flexible standard for biological part assembly. Journal of Biological Engineering 2010 4:1.