Composite

Part:BBa_K2996006

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-16)


pLuxR upstream of repL LuxR promotor is a key component in the quorum sensing system, we characterized luxpR-HS promotor from iGEM18_Tsinghua, a mutant that is reported to have high sensitivity and small leakage. In order to make our information storage device responding to AHL molecule, this promotor is put upstream of repL, BBa_K2996000 a P1 lytic replication element that can act on mini-F origin to increase copy number.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 450
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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