Part:BBa_K2981004
TyrR, a DNA binding protein, controls the biosynthesis of Phe and Tyr in E. coli
The biosynthesis of Phe and Tyr in E. coli is controlled by the DNA binding protein TyrR. The TyrR protein binds to the consensus DNA sequence TGTAAAN6TTTACA (referred to as the TyrR box), which is classified as strong or weak. In the presence of Phe, binding of the TyrR dimer to the strong cassette in vitro strongly induces gene expression by promoting binding of the RNA polymerase to the promoter or promoting binding of the second dimer to the adjacent weak box. In the presence of Tyr and ATP, the TyrR dimer self-associates to form a hexamer, and the hexamer binds weakly to the weak consensus of the consensus sequence, due to weak-frame-bound RNA polymerase forming an open complex or leaving the initiation Impaired ability of the child leads to inhibition of gene expression. Thereby regulating the repression of aroF, tyrP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 720
Illegal BglII site found at 1062 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 526
- 1000COMPATIBLE WITH RFC[1000]
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