Terminator

Part:BBa_K2978211

Designed by: Jose Pablo Delgado Navarro   Group: iGEM19_Costa_Rica   (2019-10-20)


Terminator for Lactobacillus spp

Description

  • The in silico sequence was obtained from the Duong et al (2010) where they tested this Rho-independent terminator and the K2978212 terminator.
  • This terminator determined with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-23.5 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong et al 2010).
  • The authors of this paper suggests that this specific terminator had the capability to be used as a bidirectional terminator.
  • The composite part of this sequence is present in K2978203

Terminator.png


Secondary Structure

Mfold-K2978211-1.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None