Measurement

Part:BBa_K2978205

Designed by: Jose Pablo Delgado Navarro   Group: iGEM19_Costa_Rica   (2019-10-20)


Terminator NisA Measurement in Lactobacillus spp

This part is a sample for the efficiency terminators assay, where we tested three different sequences for Lactobacillus spp K2978201 K2978202 K2978203 K2978204 and K2978205.

The composite part is based in constitutively express a GFP before of each terminator tested, and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None