Composite

Part:BBa_K2969045

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-16)


pTetO(wt)-mfLon

This part contains the coding gene of the protease mf-Lon under the promoter pTet. When there exists the transcription factor TetR, the transcription of mf-Lon will be inhibited. Mf-Lon is a protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. So if a protein contains mf-Lon degradation tag, mf-Lon will recognize the protein and degrade it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2464
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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