Composite
Part:BBa_K2969045
Designed by: Liangchen Zhuo Group: iGEM19_UCAS-China (2019-10-16)
pTetO(wt)-mfLon
This part contains the coding gene of the protease mf-Lon under the promoter pTet. When there exists the transcription factor TetR, the transcription of mf-Lon will be inhibited. Mf-Lon is a protease from Mesoplasma florum bacteria (called mf-Lon for short). While it is evolutionarily related, and mechanistically similar to the E. coli Lon protease, mf-Lon is unable to recognize E. coli degradation tags (commonly known as ssRA tags), and vice versa. So if a protein contains mf-Lon degradation tag, mf-Lon will recognize the protein and degrade it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2464
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |