Coding

Part:BBa_K2969029

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-15)


sfGFP-Asn39-Tyr151-Tyr181

sfGFP-Asn39-Tyr151-Tyr181 is transformed from the reporting part sfGFP. The asparagine residue at 39 site and tyrosine residue at 151 site and 181 site are all mutated to TAG codon.


Characterization

We inserted different number of TAG codon to sfGFP at different site to see the effect of TAG codon to the expression of sfGFP. Figure 1 shows that TAG codon can inhibit the transcription of sfGFP effectively.

Figure 1:The fluorescence of sfGEP inserted different number of TAG codon

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None