Coding
Part:BBa_K2969029
Designed by: Liangchen Zhuo Group: iGEM19_UCAS-China (2019-10-15)
sfGFP-Asn39-Tyr151-Tyr181
sfGFP-Asn39-Tyr151-Tyr181 is transformed from the reporting part sfGFP. The asparagine residue at 39 site and tyrosine residue at 151 site and 181 site are all mutated to TAG codon.
Characterization
We inserted different number of TAG codon to sfGFP at different site to see the effect of TAG codon to the expression of sfGFP. Figure 1 shows that TAG codon can inhibit the transcription of sfGFP effectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |