Coding

Part:BBa_K2969028

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-15)


sfGFP-Asn39-Tyr151

sfGFP-Asn39-Tyr151 is transformed from the reporting part sfGFP. The asparagine residue at 39 site and tyrosine residue at 151 site are both mutated to TAG codon.


Characterization

We inserted different number of TAG codon to sfGFP at different site to see the effect of TAG codon to the expression of sfGFP. Figure 1 shows that TAG codon can inhibit the transcription of sfGFP effectively.

Figure 1:The fluorescence of sfGEP inserted different number of TAG codon

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None