Part:BBa_K2951006

LysRS fusion protein

Usage and Biology

This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI(NC000913.3). LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.

Characterization

This part is part of the composite part: BBa_K2951008 and further characterized.

References

1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 275
  Illegal NotI site found at 1256
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 864
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]