Part:BBa_K2922044
Co-expression system of BBa_K346030 and BBa_K2922042 for enhancing lead absorption
Summary
This part was constructed for the cooperation of CAV1 and fusion protein DsbA-MBP. CAV1 and DsbA-MBP was connected in the same circuit to improve the absorption ability of E. coli toward lead in the aqueous environment through enrichment.
Characterization
Similar to the description in BBa_K2922043, ICP-MS was used to accurately determine the amount of lead absorption per unit mass of cells. The amounts of lead absorbed by the bacteria with DsbA-MBP and DsbA-MBP-CAV1 were measured and compared. The relative lead absorption capacity after incubation in medium with 50 μM Pb(II) was shown in Fig.2. The relative absorption capacity of DsbA-MBP-CAV1 increased significantly and was much higher than that of DsbA-MBP. At the same time, the death and rupture of bacteria caused by the toxicity of Pb(II) may be the reason for absorption capacity decreased at 18 h in DsbA-MBP group.
Conclusion
The results demonstrated that the introduction of CAV1 could not only enhance the lead absorption efficiency, but also improve the resistance of bacteria against heavy metal.
Reference
1. J. Shin et al., Display of membrane proteins on the heterologous caveolae carved by caveolin-1 in the Escherichia coli cytoplasm. Enzyme Microb Technol 79-80, 55-62 (2015).
2. J. Shin et al., Endocytosing Escherichia coli as a Whole-Cell Biocatalyst of Fatty Acids. ACS Synthetic Biology 8, 1055-1066 (2019).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 520
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 520
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 308
Illegal XhoI site found at 1005 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 520
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 520
Illegal AgeI site found at 140 - 1000COMPATIBLE WITH RFC[1000]
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