Coding

Part:BBa_K2906023

Designed by: Sara ElSafadi   Group: iGEM19_Manchester   (2019-10-15)


Ech

fcs from Pseudomonas putida KT2440 encodes enoyl-CoA hydratase (ECH) which converts feruloyl-CoA to vanillin, the fifth and final step in vanillin biosynthesis from a tyrosine precursor. The gene is codon optimised for expression in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
    Illegal NgoMIV site found at 241
    Illegal AgeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


References:

Ni, J., Tao, F., Du, H. and Xu, P. (2015). Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources. Scientific reports, 5: 13670. DOI: https://doi.org/10.1038/srep13670.


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