Coding

Part:BBa_K2906021

Designed by: Sara ElSafadi, Ewan Egan   Group: iGEM19_Manchester   (2019-10-15)


Fcs

fcs from Pseudomonas putida KT2440 encodes feruloyl-CoA synthase (FCS). It converts caffeic acid to ferulic acid, the fourth step in vanillin biosynthesis from a tyrosine precursor. The gene is codon optimised for expression in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 43
    Illegal XhoI site found at 1153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 30
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 772
    Illegal NgoMIV site found at 1027
    Illegal NgoMIV site found at 1090
    Illegal NgoMIV site found at 1107
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 802


References:

Ni, J., Tao, F., Du, H. and Xu, P. (2015). Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources. Scientific reports, 5: 13670. DOI: https://doi.org/10.1038/srep13670.


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