Regulatory

Part:BBa_K2906019

Designed by: Sara ElSafadi   Group: iGEM19_Manchester   (2019-10-15)


T7 promoter- lac operator

T7 promoter is a strong leaky promoter. Here, it is fused to the lac operator which requires a lac repressor (lacI) gene and is induced by IPTG. This sequence is found on pETM-11 and pETDuet-1 plasmids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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