Tag

Part:BBa_K2906007

Designed by: Elisa Barrow, Thomas Harrison   Group: iGEM19_Manchester   (2019-10-14)


Hydrophobic peptide - YYYPP

The hydrophobic tag was adapted from Y3P2‐GFPuv consistent of YYYPP (where Y indicates tyrosine and P proline). This tag was originally used for chromatography during protein separation. However, we decided to adapt this to enable our coloured proteins to embed in the hydrophobic beta-layer of the cortex of hair (Fexby et al., 2004).

Fexby, S., Nilsson, A., Hambraeus, G., Tjerneld, F. and Bülow, L. (2004) ‘Partitioning and characterization of tyrosine-tagged green fluorescent proteins in aqueous two-phase systems.’, Biotechnology progress, 20(3), pp. 793–8. doi: 10.1021/bp034177j.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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