Regulatory

Part:BBa_K2886010

Designed by: Leijie Dai   Group: iGEM18_Fudan-CHINA   (2018-10-07)


tet binding site

TetR is a kind of repressible promoter commonly used in synthetic biology. This time, we designed a mutation of TetR form Part:BBa_R0040 named pTight and according to the simulative result, the pTight shows a higher bind affinity compared to the original TetR.

Usage and Biology

BBa_K2886010 is a repressible tet binding site improved from BBa_R0040, a commonly used part in iGEM. This time, we designed a mutation of tet binding site. According to the simulative result, BBa_K2886010 shows a higher bind affinity compared to the original TetR. Figure 6 exhibits the result.

Figure 1: The structure and ik_ball _wtd of wild type and mutant type tet binding site. (a) Structure of wild type (WT) tet binding site. (b) Structure of mutant type (MT) tet binding site. The transcription factor is colored to show its electrostatics distribution. Compared to WT, MT with a substitution of A to C on base pair 11 has a lower interface energy decreasing from-37.467 to -38.411 kcal/mol, tested by RosettaDock. (c) The most significant improvement is lk_ball_wtd (also known as orientation-dependent solvation energy), indicating MTmay have a higher affinity to the transcriptional factor.


Improved from a previous part

Part:BBa_R0040 is a commonly used part in iGEM which has over 1000 uses till 2017. And this year, Fudan-CHINA makes a mutation to Part:BBa_R0040 to improve its combination with the tTA transcriptional factor.

For more detailed information, please click here[[1]] to read our wiki.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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