Composite

Part:BBa_K2843000

Designed by: Camille Lorie   Group: iGEM18_East_Chapel_Hill   (2018-10-07)


Fluoride RBS pro_J23

This part is an improvement of the 2017 East Chapel Hill iGem "CHOP" operon. At fluoride concentrations of 2uM and above, the delta-CRCB E coli strain (strain JW0619-1 from the Yale Coli Genetics Stock Center) will grow on chloramphenicol. This vector is designed with Xhol cutting sites so one may easily remove and replace different segments of the operon to screen for combinations of riboswitch and promoter with highest effectivity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 83
    Illegal XhoI site found at 238
    Illegal XhoI site found at 279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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