Regulatory

Part:BBa_K284008

Designed by: Fabiana de Melo Duarte, Leonardo Minete, Maria Carolina Grassi and Lucas P. Parizzi   Group: iGEM09_UNICAMP-Brazil   (2009-08-06)


tra operon promoter (Py)

Py is the tra operon promoter of F-plasmid. It is repressed by the host nucleoid-associated protein, H-NS and activeted by TraJ protein. Conflicting evidence suggests that TraY can act as both an activator and a repressor. The tra operon encodes F-plasmid proteins (except TraJ and TraM) involved in DNA transfer by conjugation in E.coli. The regions shows intrinsic curvature, which could interfere in regulation. For this reason, the two closer bends upstream promoter region are included.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
Parameters
None