Regulatory region derived from the ribitol-responsive operon
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 57
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The part is GoldenGate compatible and so has 5' and 3' sticky ends of 4bases to enable the part to ligate into pGBA2. It also has short 3 nucleotide sequences at each end, that when ligated into the pGBA2 plasmid, creates a recognition site for the Type II S restriction enzyme, BsaI.
Ordered as a Gblock from IDT. Organism TBD.