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Applications of BBa_K2810009
The Cardiff iGEM team of 2018 used this part for quantification promoter sequences in Nicotiana benthamiana, and proved that the part worked in bacteria too. We found that expression in plants was very strong and fairly consistent, making it an excellent reporter gene for use in plants. It was also used to see if the ribitol operon control unit (from E. coli) functioned in plants. Figure 1 shows the results and quantification report when being used to compare the 35S and RTBV promoters. Figure 2 shows the functioning sequence in bacteria (top left) and the use for our collaboration with WashU to characterise their ribitol control operon.