RNA

Part:BBa_K2810006

Designed by: Geraint Parry   Group: iGEM18_Cardiff_Wales   (2018-08-28)

Complimentary sequence from 5' region of the BCR3 gene

BCR3 is a predicted secreted protein that localizes to aphids bacteriocyte cells.

This sequence is taken from the complement strand from a 5’ region of the Myzus persisae BCR3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.

BCR3_5_3 is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the Phytobrick standard.

It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in BBa_K2810005.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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