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Applications of BBa_K2810002
The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the 35S CaMV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that the RTBV promoter increased expression levels to 10-100x that of background levels. This is still less than 35S CaMV promoter by a factor of 10-100.
We also performed an assay using eGFP to quantify this promoter. We used the RTBV promoter as it is supposed to have higher expression in vascular tissue, which Figure 3 shows. However, the raw quantification report did not show that it had significantly higher expression than control leaves, even though the values were higher.