RBS

Part:BBa_K2782000:Design

Designed by: Dan Zhou   Group: iGEM18_BUCT-China   (2018-08-15)


Ribosome binding site preceding the repressor protein sequence of the fatty acid operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note the distance between the control RBS and the start codon ATG. Retain the consensus sequence of the ribosome binding site - AGGAGG-



Source

Integration expression vector pSe1Bb1s-gfp, complete sequence Sequence ID: KJ814971.1

References