RBS

Part:BBa_K2765024:Design

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-30)


1000bp Homologous arm+ura(△yno1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 668
    Illegal XhoI site found at 535
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 868
    Illegal BsaI site found at 1669
    Illegal BsaI site found at 2699
    Illegal SapI.rc site found at 1516
    Illegal SapI site found at 2304


Design Notes

cloned the upstream homologous arm and the downstream homologous arm of yno1 from the genome of CEN.PK2-1C and cloned Leu marker from pESC-Leu.ligased Leu marker and the complete fragment by OE-PCR then transformed  them into CEN.PK2-1C(△yno1).



Source

S. cerevisiae CEN.PK2-1C


References