RBS

Part:BBa_K2761006:Design

Designed by: Alan Eduardo Ávila Ramírez   Group: iGEM18_Tec-Monterrey   (2018-10-10)


RBS for Cas1-Cas2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has been used for E. coli BL21 D3


Source

Was obtained from James K. Nunez

References

Nuñez, J. K., Kranzusch, P. J., Noeske, J., Wright, A. V., Davies, C. W., & Doudna, J. A. (2014). Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity. Nature structural & molecular biology, 21(6), 528.