Vio operon ABCE
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1029
- 21Illegal BamHI site found at 4289
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 3814
Illegal AgeI site found at 4010
- 1000COMPATIBLE WITH RFC
The origonal vio operon was designed with the vioD gene flanked by complementary sticky end restriction sites for BglII and BclI. This allowed the vioD to be easily removed, leading to the production of the light green pigment.
This part was made from the vio operon biobrick, part number BBa_K274002. The vioD gene has been removed by restriction digest.