FabI expressed under high constitutive expression cassette
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 470
Illegal NheI site found at 493
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC
Various combinations of promoters and ribosome binding sites were tested to see if triclosan resistance could be conferred. K314100 was the only part which gave sufficiently high fabI expression for resistance.
FabI was PCR amplified from the genome of E. coli DH5-alpha to add the Biobrick prefix and suffix. K314100 was taken from the 2018 iGEM distribution kit.