RNA

Part:BBa_K2715015

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


Antisense RNA targeting tcdA 1

Usage

This part is an antisense RNA which targets the Clostridium difficile toxin TcdA. It has been used in the composite part BBa_K2715007.


Biology

The antisense targets 24bp of the coding sequence of C. difficile TcdA as well as the upstream region to include the ribosome binding site.

BBa K2715015 pic.png

Characterisation

This part was characterised as part of a composite part BBa_K2715007 in terms of its ability to reduce the cytotoxicity of C. difficile supernatant. The composite part was effective in reducing toxin production by 80%.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None