Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The sequence was codon optimized with the codon optimization tool provided by IDT.
Biobrick was ordered as a customized genes from Integrated DNA Technologies (IDT)
constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).