Signalling

Part:BBa_K266006

Designed by: UNAM FCIENCIAS-CINVESTAV IRA   Group: iGEM09_IPN-UNAM-Mexico   (2009-10-13)


PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP

BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1492


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//regulation/negative
//regulation/multiple
//function/cellsignalling/LasR
//function/cellsignalling/LuxR
Parameters
negative_regulators1
positive_regulators1