Terminator

Part:BBa_K2656026:Design

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-20)


Double transcriptional terminator (B0010-B0012)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has been designed adding to the terminator sequence: GCGCCGTCTCGCTCGGCTT upstream and CGCTTGAGCGAGACGGCGC downstream. These sequences allow the assembly of the part into BBa_P10500 with a Golden Gate reaction using BsmbI endonuclease.

Source

The original sequence has been obtained from the Registry of Standard Biological Parts and it has been synthetized by IDT.


References