Very Low Ribosome Binding Site J61101
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
It has been designed adding to the RBS sequence: GCGCCGTCTCGCTCGTACT upstream and tactagAATGTGAGCGAGACGGCGC downstream. These sequences allow the assembly of the part into BBa_P10500 with a Golden Gate reaction using BsmbI endonuclease. We have added the equivalent of the BioBrick scar to the RBS sequence because we have noted that without it, the transcriptional units with this RBS had not expression.
The original sequence has been obtained from the Registry of Standard Biological Parts and it has been synthetized by IDT.