Composite

Part:BBa_K2652001

Designed by: Siyun Xiong   Group: iGEM18_HBUT-China   (2018-10-08)


promotor, RBS, and terminator

This section contains the promoter, RBS, BamHI restriction sites and terminators. We cut this part with the enzyme BamHI and inserted the nickel ion channel gene NikABCDE into it using the Gipson clone protocol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None