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Part:BBa_K265008

Designed by: Timothy Fenton   Group: iGEM09_UC_Davis   (2009-07-08)

Ice Nucleation Protein NC

The ice-nucleation protein (INP) from Pseudomonas syringae, is used by its natural host to nucleate ice formation and is implicated in P. syringae associated pathogenesis. INP and a truncated derivative lacking the central domain (INPNC) have been used extensively for displaying proteins on the surface of E. coli (1,3). For instance, carboxymethylcellulase and AldO have been successfully displayed on the surface of E. coli using INPNC (1, 3).

Park et al. have shown that INPNC when fused to the phaZ1 gene, including its signal sequence, can serve as a suitable surface delivery and secretion device of the otherwise toxic phaZ1 gene product (2).

This part was synthesized by Mr. Gene with codon optimization and subsequently transferred into vector pSB1AK3. As it is expected that this part will be used in the context of the fusion protein, the prefix and suffix for this part are consistent with the BBF RCF-12 standard.

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We have proposed to build and test a general protein secretion system modeled after that developed by Park et al. in which a fusion of INPNC and the signal sequence from the phaZ1 gene are used to secrete any target protein.


We have modified this protein to be consistent with BBF RFC-12 Standard. We have submitted this part to the parts registry as part BBa_K265008.

This part is authored by Richard Hauser, Tim Fenton, Willis Wong, Lydia Kwon, Jimmy Huang, and Pantea Gheibi. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aIce Nucleation Protein NC