DNA

Part:BBa_K2635008

Designed by: Hsuan Cheng, Ching-Lin Kao, Yi-Chien Chuang   Group: iGEM18_NTHU_Formosa   (2018-10-03)


TEV cleavage site ENLYFQG

TEV proteases recognize a linear epitope composed of 7 amino acids and cut the linkage between the last and the second-last amino acid. The high specificity of cutting amino acid linkages makes it a popular tool for direct expression in living cells and protein purification. In our design, we use ENLYFQL at TEV N-terminal and ENLYFQG at TEV C-terminal as cutting site according to Baeumler’s paper in 2017.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Baeumler, T. A., Ahmed, A. A., &Fulga, T. A. (2017). Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Reports, 20(11), 2639–2653. https://doi.org/10.1016/j.celrep.2017.08.044
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