Part:BBa_K2617010

FRE_adhE: The fermentation regulatory element of Escherichia coli controls the expression

Fre_adhE is the fermentation control element of Escherichia coli controls the expression,including the promoter, ribosome binding site (RBS) and the entire transcription factor (TF) binding site.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

FRE_adhE is the transcriptional and translational regulatory element which contains the cassette of 5′ upstream region controlling the expression of the major fermentative gene adhE. For the function determination, we used the FRE_adhE to drive the synthetic 1-butanol production. And the plasmid piGEM2018-Module002 was constructed successfully(Fig. 1).

Fig. 1 piGEM2018-Module002

Theoretically, fermentation of 1-butanol will be triggered under anaerobic conditions because of FRE’s regulation. We detected the production of butanol in E. coli BL21(DE3) carrying the above plasmid by gas chromatography.

Fig. 2 The production of butanol determined using GC.

The result (Fig. 2) shows our fermentation regulatory element successfully worked.