Part:BBa_K2606003

cspD2

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The cspD2 gene is a reported that can express cold shock protein in Lactococcus lactis, and can help bacteria survive at low temperature.

Kegg number: LLNZ_06470

https://www.genome.jp/dbget-bin/www_bget?lln:LLNZ_06470

Fig 1 Electron microscopy before and after repeated freezing and thawing.

Before freezing and thawing, the cell structure of the control strain and the recombinant strain remained intact. After repeated freezing and thawing for 4 times, the cell membrane of the cell became thin and rough, and some intracellular substances overflowed. In comparison, the cell structure of the recombinant strain remains more intact, and the damage of the cell membrane is alleviated, thereby effectively reducing the damage caused by freezing stress on the cells.

Source

We obtained the sequence from KEGG library. CspD2 is a fragment divided from Lactococcus lactis.

Kegg number: LLNZ_06470. https://www.genome.jp/dbget-bin/www_bget?lln:LLNZ_06470

References

1. Yinghua Zhang, Yuting Lei, Guicheng Huo. 2008. Expression of cold-shock-protein genes from Lactococcus lactis and analysis of the cryoprotection function. Acta Microbiologica Sinica 48(9)：1203~1207.

2. Ermolenko DN, Makhatadze GI. Bacterial cold-shock proteins. Cell Mol Life Sci, 2002, 59: 1902−1913