Composite
NOTMlcRE-A

Part:BBa_K2588024

Designed by: Dominika Wawrzyniak, Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)


3-methyl-1-butanol synthesis module, glucose-induced

This part consists of the genes LeuABCD, kivD and ADH2. It produces 3-methyl-1-butanol according to the pathway seen in fig. 1.1

Fig 1: Metabolic pathway for amino acid biosynthesis and 3-methyl-1-butanol production. All genes are from E. coli unless otherwise noted. BS B. subtilis, LL L. lactis, SC S. cerevisiae.1

References

  1. Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86, 1155–1164 (2010).

      2. Sequence and Features


      Assembly Compatibility:
      • 10
        COMPATIBLE WITH RFC[10]
      • 12
        INCOMPATIBLE WITH RFC[12]
        Illegal NheI site found at 144
        Illegal NheI site found at 167
      • 21
        INCOMPATIBLE WITH RFC[21]
        Illegal BamHI site found at 7747
        Illegal XhoI site found at 570
      • 23
        COMPATIBLE WITH RFC[23]
      • 25
        INCOMPATIBLE WITH RFC[25]
        Illegal NgoMIV site found at 1328
        Illegal AgeI site found at 6485
      • 1000
        INCOMPATIBLE WITH RFC[1000]
        Illegal SapI site found at 7573
        Illegal SapI.rc site found at 4405


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