Part:BBa_K2588012

Acetyl-CoA biotin carboxylase

Usage and Biology

The Acetyl-CoA biotin carboxylase is part of the acetyl-CoA carboxylase ACC, which is the major regulator for fatty acid biosynthesis in E. coli. It is encoded as a single mRNA with the upstream accB gene, which is encoding for the biotin carboxyl carrier protein of the complex.¹ The acetyl-CoA biotin carboxylase catalyzes the reaction of biotin to carboxybiotin by forming carboxyphosphate out of ATP and bicarbonate and then transferring the carboxyl group from carboxyphosphate to the biotin, which is bound to the biotin carboxyl carrier protein.²

The acetyl-CoA biotin carboxylase was used in our myristic acid synthesis module, BBa_K2588022.

References

1. Li, S. J. & Cronan, J. E. The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267, 855–63 (1992).
2. Broussard, T. C., Price, A. E., Laborde, S. M. & Waldrop, G. L. Complex formation and regulation of escherichia coli acetyl-CoA carboxylase. Biochemistry (2013). doi:10.1021/bi4000707

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 918
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]