Coding

Part:BBa_K2583000:Design

Designed by: LiuJie   Group: iGEM18_Tsinghua-A   (2018-10-03)


HRH4_CDS

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gaattc will be mutated to gagttc to avoid the restriction enzyme digestion site of EcoRI


Source

Homo sapiens:GRCh38.p12 (GCF_000001405.38)Chr 18 (NC_000018.10):24,457,882 - 24,490,113 Sequence found through GeneBank https://www.ncbi.nlm.nih.gov/gene/59340

References