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Applications of BBa_K257021
Measurement of BBa_K257021 delay
BBa_K257021 was grown overnight in LB medium with:
- Glucose 1 %
- IPTG (0.2mg/ml)
In the second graph, fluorescence was normalized by the mean Fluo/OD of all the conditions where mRFP1 is continuously expressed.
We observe a delay of ~10000sec between the beginning of the experiment and the changes in RFP expression.
Note that the delay is shorter for the LB + glucose -> LB + IPTG shift than for the LB + glucose -> LB shift.
In presence of glucose pLac is repressed and then in absence of TetR the pTet promoter is turned on and activates the transcription of mRFP. Accordingly to the construction more bacteria are fluorescent in presence of glucose than in the control.